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DescriptionThe intronless gene for SOX2 encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
StorageAliquot the SOX-2 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
NotesTitration of the SOX-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
ImmunogenA portion of amino acids 89-119 from the human protein was used as the immunogen for this SOX-2 antibody.
Recommended dilutionsWestern blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
Intented useThis SOX-2 antibodyis to be used only for research purposes and not for diagnostics..
ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Tested applicationsWB, IHC, IF, FACS, ELISA
ClonePolyclonal antibody
French translationanticorps
Uniprot #P48431
Recognised antigenSOX-2