| Properties |
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes. |
| Antigen background |
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. |
| Synonyms |
ICA 12; ICA12; Islet cell antibody 12; Islet cell antigen 12; MGC117216; SOX 13; SOX 13 protein; sox13; SOX13 protein; SOX13_HUMAN; SRY Sex determining region Y-box 13; SRY box 13; SRY box containing gene 13; SRY related HMG box gene 13; SRY sex determining region Y box 13; Transcription factor SOX 13; Transcription factor Sox-13; Type 1 diabetes autoantigen; Type 1 diabetes autoantigen ICA 12; Type 1 diabetes autoantigen ICA12. |
| Storage conditions |
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year. |
| Crossreactive species details |
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known. |
| Source |
KLH conjugated synthetic peptide derived from human ICA12 |
| Specificity |
This is a highly specific antibody against ICA12/SOX13. |
| Long name |
ICA12/SOX13 Polyclonal Antibody, FITC Conjugated |
| Purification method |
This antibody was purified via Protein A. |
| Host Organism |
Rabbit (Oryctolagus cuniculus) |
| Category |
Conjugated Primary Antibodies |
| Clone |
Polyclonal antibody |
| Recommended dilutions |
IF(IHC-P)(1:50-200) |
| Additional conjugation |
Anti-FITC Antibody |
| Crossreactivity |
Human, Mouse, Rat |
| Concentration |
1ug per 1ul |
| Target Antigen |
ICA12/SOX13 |
| Excitation Emission |
494nm/518nm |
| Clonality |
Polyclonal |
| Modification |
Unmodified |
| Tested applications |
IF(IHC-P) |
| French translation |
anticorps |
| Modification site |
None |
| Conjugation |
FITC |
