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Description Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Alternate gene name ANOP3, MCOPS3, MGC2413, SRY-related HMG-box gene 2|sex-determining region Y-box 2|transcription factor SOX2
ELISA's specificity To know the specificity of this test please feel free to contact us
QC The kit is manufactured at ISO 9001 certified facilities.
Notes For research use only. Not for diagnostic procedures.
Protocol Please see ELISA's datasheet, otherwise contact us
Assay principle Please see ELISA's datasheet, otherwise contact us
Precision of the test Please see ELISA's datasheet, otherwise contact us
ELISA's stability Please see ELISA's datasheet, otherwise contact us
Samples to be analyzed serum, plasma, tissue homogenates, cell lysates
Gene name SRY (sex determining region Y) -box 2
Research main area Epigenetics and Nuclear Signaling
Alternate protein number Please refer to SwissProt
Gene number Please refer to GenBank
Detection limits 31.25 pg/ml-2000 pg/ml
Estimated production time 7-11 business days
Assay class Please contact us
Latin name Mus musculus
ELISA detection Colorimetric
Sensitivity limit 7,81 pg/ml
Assay duration 1-5 hours
Use for 6 months
Shipping requirements Blue ice
Protein number P48432
Verified applications ELISA
Verified reactivity Mouse