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NameDescription
PropertiesIf you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
Antigen backgroundSox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
SynonymsICA 12; ICA12; Islet cell antibody 12; Islet cell antigen 12; MGC117216; SOX 13; SOX 13 protein; sox13; SOX13 protein; SOX13_HUMAN; SRY Sex determining region Y-box 13; SRY box 13; SRY box containing gene 13; SRY related HMG box gene 13; SRY sex determining region Y box 13; Transcription factor SOX 13; Transcription factor Sox-13; Type 1 diabetes autoantigen; Type 1 diabetes autoantigen ICA 12; Type 1 diabetes autoantigen ICA12.
Storage conditionsKeep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Crossreactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
SourceKLH conjugated synthetic peptide derived from human ICA12
SpecificityThis is a highly specific antibody against ICA12/SOX13.
Long nameICA12/SOX13 Polyclonal Antibody, FITC Conjugated
Purification methodThis antibody was purified via Protein A.
Host OrganismRabbit (Oryctolagus cuniculus)
CategoryConjugated Primary Antibodies
ClonePolyclonal antibody
Recommended dilutionsIF(IHC-P)(1:50-200)
Additional conjugationAnti-FITC Antibody
CrossreactivityHuman, Mouse, Rat
Concentration1ug per 1ul
Target AntigenICA12/SOX13
Excitation Emission494nm/518nm
ClonalityPolyclonal
ModificationUnmodified
Tested applicationsIF(IHC-P)
French translationanticorps
Modification siteNone
ConjugationFITC